Determination of flufenamic acid and mefenamic acid in pharmaceutical preparations and biological fluids using flow injection analysis with tris(2,2 '-bipyridyl)ruthenium(II) chemiluminescence detection
Fa. Aly et al., Determination of flufenamic acid and mefenamic acid in pharmaceutical preparations and biological fluids using flow injection analysis with tris(2,2 '-bipyridyl)ruthenium(II) chemiluminescence detection, ANALYT CHIM, 416(1), 2000, pp. 87-96
A novel chemiluminescent method using flow injection has been investigated
for the rapid and sensitive determination of flufenamic and mefenamic acids
. The method is based on a tris(2,2'-bipyridyl)ruthenium(III) chemiluminesc
ence reaction. Ru(bipy)(3)(3+) is chemically generated by mixing two stream
s containing solutions of tris(2,2'-bipyridyl)ruthenium(II) and acidic ceri
um(IV) sulphate. After selecting the best operating parameters calibration
graphs were obtained over the concentration ranges 0.07-6.0 and 0.05-6.0 mu
g ml(-1) for flufenamic acid and mefenamic acid, respectively. The Limits
of detection (s/n = 3) were 3.6 x 10(-9) M flufenamic acid and 2.1 x 10(-7)
M mefenamic acid. The method was successfully applied to the determination
of these compounds in pharmaceutical formulations and biological fluids. A
proposal for the reaction pathway was given. (C) 2000 Elsevier Science B.V
, All rights reserved.