Detection of atrazine based on the SPR determination of P450 mRNA levels in Saccharomyces cerevisiae

Authors
Lim, TK Oyama, M Ikebukuro, K Karube, I
Citation
Tk. Lim et al., Detection of atrazine based on the SPR determination of P450 mRNA levels in Saccharomyces cerevisiae, ANALYT CHEM, 72(13), 2000, pp. 2856-2860
Citations number
26
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
00032700 → ACNP
Volume
72
Issue
13
Year of publication
2000
Pages
2856 - 2860
Database
ISI
SICI code
0003-2700(20000701)72:13<2856:DOABOT>2.0.ZU;2-4
Abstract
We describe a novel method for quantification of atrazine based on detectio n of P450 mRNA levels in Saccharomyces cerevisiae. The selected oligonucleo tide probe exhibited specificity against P450 mRNA and was successfully imm obilized on the sensor chip. The mRNA was subsequently quantified by RU cha nge using a SPR system. When the cells were disrupted by boiling, mRNA coul d be measured without further purification at reduced sensitivity. This sim ple technique permits the detection of atrazine within 15 min. This rapid a nd highly sensitive method can be used for the detection of atrazine.