Vg. Nielsen et al., Evaluation of the contribution of platelets to clot strength by thromboelastography in rabbits: The role of tissue factor and cytochalasin D, ANESTH ANAL, 91(1), 2000, pp. 35-39
Citations number
10
Categorie Soggetti
Aneshtesia & Intensive Care","Medical Research Diagnosis & Treatment
The contribution of platelets and soluble clotting components to clot stren
gth has been the focus of several clinical studies using thromboelastograph
y; it would, therefore, be beneficial to develop an animal model with which
to mechanistically approach hemostatic disorders. Thus, we proposed to det
ermine if the contribution of platelet function (G(P), dyne/cm(2)) and solu
ble components of the coagulation pathway to total clot strength (G(T)) in
rabbits were similar to those in humans. Blood was sampled from the ear art
eries of conscious rabbits (n = 12); 350 mu L of the blood was placed in a
thromboelastograph. Ten microliters of normal saline, cytochalasin D (an in
hibitor of microtubule function, 10 mu M final concentration), or tissue fa
ctor (a potent stimulator of platelet function, 0.00625% final concentratio
n) was added to the blood sample, and thromboelastography performed for 1 h
. The G(T) (mean +/- SD) was significantly (P < 0.001) different among samp
les exposed to normal saline, cytochalasin D, or tissue factor, with G(T) v
alues of 7238 +/- 1432, 937 +/- 372, and 16,556 +/- 3314, respectively. G(P
) was responsible for 87% and 94% of G(T) in the absence or presence of tis
sue factor, respectively. G(T) did not significantly correlate with platele
t concentration in the absence or presence of tissue factor. The contributi
on of G(P) to G(T) is similar to that observed in humans.