Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL60 cells

The expression of human purinergic P2 receptors (P2X1-7 and P2Y1-11) as wel l as the ecto-enzymes apyrase (CD39) and 5'-nucleotidase (CD73) was investi gated on the nucleic acid level during granulocytic and monocytic different iation of HL60 cells and on peripheral human blood leukocytes. RT-PCR and d ot-blot hybridization assays indicated that mRNA transcripts of all analyze d P2 receptors apart from the P2X3 receptor were expressed during myeloid d evelopment of HL60 cells, showing a distinct regulation during the course o f differentiation. In blood leukocytes, transcripts of P2X5, P2X7 and all P 2Y receptors, except for P2Y6, receptor were found. CD39 and CD73 showed a marked upregulation during myeloid maturation. Functional analysis of P2 re ceptor-mediated intracellular Ca2+-increase after stimulation with ATP reve aled no change during granulocytic differentiation, but showed a strong att enuation in both potency and efficacy during monocytic development of HL60 cells. (C) 2000 Published by Elsevier Science B.V.