Citation
V. Schauer-vukasinovic et al., Cloning, expression and functional characterization of rat napsin, BBA-GENE ST, 1492(1), 2000, pp. 207-210
Citations number
10
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781
→ ACNP
Volume
1492
Issue
1
Year of publication
2000
Pages
207 - 210
Database
ISI
SICI code
0167-4781(20000621)1492:1<207:CEAFCO>2.0.ZU;2-N
Abstract
A full-length cDNA clone coding for rat napsin was identified by homology s
earch of the ZooSeq rat EST database (Incyte). Northern blot analysis revea
led high expression of napsin mRNA transcripts in kidney, lung and spleen.
Western blot analysis showed that rat napsin is expressed in kidney as a 50
-kDa, highly glycosylated, monomeric protein. Lysates prepared from human e
mbryonic kidney cells (HEK293) transfected with rat napsin showed increased
enzymatic activity which was inhibited by pepstatin. (C) 2000 Elsevier Sci
ence B.V. All rights reserved.