Molecular cloning of cDNA encoding a ubiquitin-activating enzyme (E1) fromgoldfish (Carassius auratus) and expression analysis of the cloned gene

Destruction of cyclin B is required to the mitotic and meiotic cycles. A cy clin-specific ubiquitinating system, including ubiquitin-activating enzyme (E1), is thought to be responsible for cyclin B destruction. Here we presen t the cloning, sequencing and expression analysis of goldfish, Carassius au ratus, E1 from goldfish ovary. The cloned cDNA is 4069 bp long and encodes 1059 amino acids. The deduced amino acid sequence is highly homologous to E 1 from other species. Recombinant goldfish E1 could transfer ubiquitin to c yclin-selective ubiquitin-conjugating enzyme. Tissue distribution revealed a single 4.0-kb message ubiquitous among tissues. (C) 2000 Elsevier Science B.V. All rights reserved.