The impacts of crop rotations and N fertilization on different pools of ure
ase activity were studied in soils of two long-term field experiments in Io
wa; at the Northeast Research Center (NERC) and the Clarion-Webster Researc
h Center (CWRC). Surface soil samples (0-15 cm) were taken in 1996 and 1997
in corn, soybeans, oats, or meadow (alfalfa) plots that received 0 or 180
kg N ha(-1), applied as urea before corn and an annual application of 20 kg
P and 56 kg K ha(-1): The urease activity in the soils was assayed at opti
mal pH (THAM buffer, pH 9.0), with and without toluene treatment, in a chlo
roform-fumigated sample and its nonfumigated counterpart. The microbial bio
mass C (C-mic) and N (N-mic) were determined by chloroform fumigation metho
ds. The total, intracellular, extracellular and specific urease activities
in the soils of the NERC site were significantly affected by crop rotation,
but not by N fertilization. Generally, the highest total urease activities
were obtained in soils under 4-year oats-meadow rotations and the lowest u
nder continuous corn. The higher total activities under multicropping syste
ms were caused by a higher activity of both the intracellular and extracell
ular urease fractions. In contrast, the highest values for the specific ure
ase activity, i.e. of urease activity of the microbial biomass, were found
in soils under continuous soybean and the least under the 4-year rotations.
Total and extracellular urease activities were significantly correlated wi
th C-mic (r>0.30* and >0.40**) and N-mic (r>0.39** and >0.44**) in soils of
the NERC and CWRC sites, respectively. Total urease activity was significa
ntly correlated with the intracellular activity (r>0.73***). About 46% of t
he total urease activity of the soils was associated with the microbial bio
mass, and 54% was extracellular in nature.