Detection of protease activity with a fluorescence-labelled peptide substrate on a TLC plate

Citation
T. Uchikoba et al., Detection of protease activity with a fluorescence-labelled peptide substrate on a TLC plate, BIOS BIOT B, 64(6), 2000, pp. 1285-1287
Citations number
6
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
6
Year of publication
2000
Pages
1285 - 1287
Database
ISI
SICI code
0916-8451(200006)64:6<1285:DOPAWA>2.0.ZU;2-T
Abstract
A small amount of peptidase activity could be detected using an amine deriv atizing reagent, fluorescein isothiocyanate (FITC), which has been used to produce a fluorogenic peptide. The substrate produced, FITC-peptide, gave a clear spot on a silica gel sheet upon exposure to UV light. The peptidase activity of angiotensin-converting enzyme (ACE), trypsin, chymotrypsin, cuc umisin, and that of some plant tissues were detected by using a fluorogenic angiotensin I. This showed that the substrate specificity of proteolytic e nzymes can be distinguished from the others by this procedure.