Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbondioxide

Authors
Ishikawa, H Shimoda, M Yonekura, A Mishima, K Matsumoto, K Osajima, Y
Citation
H. Ishikawa et al., Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbondioxide, BIOS BIOT B, 64(6), 2000, pp. 1295-1297
Citations number
12
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
6
Year of publication
2000
Pages
1295 - 1297
Database
ISI
SICI code
0916-8451(200006)64:6<1295:UOPABM>2.0.ZU;2-V
Abstract
The conformational changes in alpha-helical poly-L-glutamic acid caused by microbubbling supercritical CO2 were investigated with circular dichroism s pectra. After microbubbling using a micropore filter at 35 and 30 MPa for 3 0 min, alpha-helix content decreased to 37%, while without the filter it wa s 68%. The alpha-helix structure was significantly decomposed by a high den sity of CO2. No important changes were observed in heating, autoclaving, or pH-lowering.