Construction of mutants of Sphingomonas paucimobilis defective in terminalmannose in the glycosphingolipid

Authors
Kaneko, A Miyadai, H Danbara, H Kawahara, K
Citation
A. Kaneko et al., Construction of mutants of Sphingomonas paucimobilis defective in terminalmannose in the glycosphingolipid, BIOS BIOT B, 64(6), 2000, pp. 1298-1301
Citations number
17
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
6
Year of publication
2000
Pages
1298 - 1301
Database
ISI
SICI code
0916-8451(200006)64:6<1298:COMOSP>2.0.ZU;2-I
Abstract
Mutants of Sphingomonas paucimobilis defective in a part of the carbohydrat e moiety of the glycosphingolipid (GSL) were constructed by transposon (Tn5 )-insertional mutagenesis. Defective mutants were selected by ELISA using t he antibody recognizing the tetrasaccharide-type GSL (GSL-4A) of S. paucimo bilis. Eight defective mutants were selected from about 8,000 kanamycin-res istant strains, and seven of them were found to lack the terminal mannose o f GSL-4A. The chemical structure of the mutant GSL was investigated, and pr oved that the rest of the structure was not changed by the mutation.