Hematopoietic progenitors from umbilical cord blood

Background/Aim: The aim of this study is to improve the obstetrician-based cord blood collection system and an efficient recovery of CD34+ haematopoie tic progenitor stem cells. Methods: CD34+ cells were purified from total bl ood using a positive selection enrichment method, called Mini-Macs. Results : The final yield of CD34+ cells we obtained was 10(4) cells/ml, with a CD3 4+ purity of 99%. Conclusion: Our results confirm that, by using this metho d, it is possible to get a significant stem cell number, thus improving tra nsplanting both peripheral stem cells and umbilical cord ones. Copyright (C ) 2000 S. Karger AG, Basel.