Viability and functional activity of cryopreserved mononuclear cells

Factors that influence viability and function of cryopreserved peripheral b lood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Cl inical Trial Units. PBMC viability ranged from I to 96% with a median of 70 %, was higher in laboratories with experienced staff, and was not significa ntly associated with CD4 cell number. Function of cryopreserved PBMC, measu red by lymphocyte proliferation, was associated with viability. Preparation s with viability greater than or equal to 70% had consistent proliferative responses and,were suitable For functional analyses.