Proteomic analysis of the atrophying rat soleus muscle following denervation

A proteomic analysis was performed comparing normal rat soleus muscle to de nervated soleus muscle at 0.5, 1, 2, 4, 6, 8 and 10 days post denervation. Muscle mass measurements demonstrated that the times of major mass changes occurred between 2 and 4 days post denervation. Proteomic analysis of the d enervated soleus muscle during the atrophy process demonstrated statistical ly significant (at the p < 0.01 level) changes in 73 soleus proteins, inclu ding coordinated changes in select groups of proteins. Sequence analysis of ten differentially regulated proteins identified metabolic proteins, chape rone and contractile apparatus proteins. Together these data indicate that coordinated temporally regulated changes in the proteome occur during dener vation-induced soleus muscle atrophy, including changes in muscle metabolis m and contractile apparatus proteins.