Jf. Quignard et al., 3-morpholinosydnonimine (SIN-1) and K+ channels in smooth muscle cells of the rabbit and guinea pig carotid arteries, EUR J PHARM, 399(1), 2000, pp. 9-16
Experiments were designed to determine the subtype of K+ channels activated
by the nitrovasodilator 3-morpholinosydnonimine (SIN-1) in smooth muscle c
ells of the rabbit and guinea pig carotid arteries. Membrane potential was
recorded in isolated segments with intracellular microelectrode and K+ curr
ents in freshly dissociated smooth muscle cells, with the patch-clamp techn
ique. In the guinea pig carotid artery, SIN-1 caused a glibenclamide-sensit
ive hyperpolarization. The nitrovasodilator did not affect the whole-cell K
+ current, but activated a glibenclamide-sensitive K+ current. In the rabbi
t carotid artery, SIN-1 induced only an iberiotoxin-sensitive repolarizatio
n in phenylephrine-depolarized tissue and in isolated cells, enhanced the a
ctivity of an iberiotoxin-sensitive K+ current. These findings demonstrate
that the population of K+ channels activated by nitric oxide (NO) is specie
s-dependent and support the conclusion that, in the guinea pig carotid arte
ry, in contrast to the rabbit carotid artery, the release of NO cannot acco
unt for the responses attributed to endothelium-derived hyperpolarizing fac
tor (EDHF). (C) 2000 Elsevier Science B.V. All rights reserved.