Neonatal gene therapy: transfer and expression of exogenous genes in neonatal sheep following direct injection of retroviral vectors into the bone marrow space

Objective. We investigated whether gene transfer into hematopoietic cells c ould be achieved by direct injection of retroviral vector supernatant into the bone marrow space of newborn sheep. Materials and Methods. Six sheep (5 weeks old) were injected bilaterally wi th either 1 mL of G1nBgSvNa8.1 vector supernatant (titer: 1 x 10(7)) in eac h hip (n = 5) or with 3 mL of the same vector preparation/hip (n = 1). In a ddition, one 3-month-old sheep was injected unilaterally with 1 mt of the s ame vector preparation. Blood and marrow of these animals were analyzed for the transgene before injection and at intervals thereafter. Results. At 1 week postinjection, an average of 11.6% of the lymphocytes an d 25.5% of the granulocytes/monocytes in the marrow, and an average of 0.9% of the lymphocytes and 1.8% of the granulocytes/monocytes in the blood con tained and expressed the LacZ gene. The presence/expression of the transgen e has persisted for at least 13 months within the blood and bone marrow of these animals. Conclusions. These findings demonstrate that the direct injection of small volumes of high-titer retroviral supernatant into the bone marrow of newbor n sheep results in transduction of hematopoietic cells that persists for at least 13 months postinjection, (C) 2000 International Society for Experime ntal Hematology. Published by Elsevier Science Inc.