Secondary structure of the 5 '-region of PGY1/MDR1 mRNA

In order to identify the optimal target sites for antisense oligonucleotide s in the human multiple drug resistance mRNA, the secondary structure of th e 5'-terminal part of this mRNA (nucleotides 1-678) was investigated. By us ing results of probing with ribonucleases T1, ONE and V1 and results of com puter simulations, a model of the 5'-region of the PGY1/MDR1 mRNA wits buil t. The molecule is formed by three major domains comprising several hairpin s separated by single-stranded fragments. The predicted single-stranded reg ions of the PGY1/MDR1 mRNA efficiently bind complementary oligonucleotides, (C) 2000 Federation of European Biochemical Societies. Published by Elsevi er Science B.V. All rights reserved.