Functional crosstalk between phospholipase D-2 and signaling phospholipaseA(2)/cyclooxygenase-2-medisted prostaglandin biosynthetic pathways

We performed reconstitution analyses of functional interaction between phos pholipase A(2) (PLA(2)) and phospholipase D (PLD) enzymes. Cotransfection o f HEK293 cells with cytosolic (cPLA(2)) or type IIA secretory (sPLA(2)-IIA) PLA(2) and PLD2, but not PLD1, led to marked augmentation of stimulus-indu ced arachidonate release, Interleukin-1-stimulated arachidonate release was accompanied by prostaglandin E-2 production via cyclooxygenase-2, the expr ession of which was augmented by PLD2. Conversely, activation of PLD2, not PLD1, was facilitated by cPLA(2) or sPLA(2)-IIA, Thus, our results revealed functional crosstalk between signaling PLA(2)s and PLD2 in the regulation of various cellular responses in which these enzymes have been implicated. (C) 2000 Federation of European Biochemical Societies. Published by Elsevie r Science B.V. All rights reserved.