Lactobacillus brevis cells at the end of growth release intracellular beta-
galactosidase into the medium. Enzyme release begins immediately after the
end of cell multiplication and is connected to cell autolysis and breakage
of the cell wall. Lactobacillus plantarum cells did not release beta-galact
osidase because autolysis occurred in a different way. The cells initially
collapsed, without showing signs of cell wall breakage, which became eviden
t only after 30 or more days from the end of growth.