YAC/BAC-based physical and transcript mapping around the gracile axonal dystrophy (gad) locus identifies Uchl1, Pmx2b, Atp3a2, and Hip2 genes

We generated a yeast artificial chromosome (YAC)/ bacterial artificial chro mosome (BAC)-based physical and transcript map of a region containing the g racile axonal dystrophy (gad) locus on mouse chromosome 5, The YAC/BAC cont ig consists of 13 YAC and 49 BAC clones onto which 4 genes, 40 expressed se quence tags, and 7 new DNA polymorphisms were ordered, Using this physical map, we mapped Uchl1 encoding ubiquitin carboxyl-terminal hydrolase I, whos e deletion has been determined to cause the god mutation. We also mapped th ree other recently identified genes: Hip2, encoding Huntingtin interacting protein 2; Atp3a2, encoding a P-type ATPase; and Pmx2b, encoding PHOX2b. (C ) 2000 Academic Press.