R. Latza et al., Platelet function in platelet concentrates during storage: Comparison of two blood cell separators, INFUS THER, 27(2), 2000, pp. 94-100
Citations number
35
Categorie Soggetti
Hematology
Journal title
INFUSION THERAPY AND TRANSFUSION MEDICINE-INFUSIONSTHERAPIE UND TRANSFUSIONSMEDIZIN
Background: In a controlled, randomized, prospective, open, comparative stu
dy we evaluated the platelet function of 112 platelet concentrates (PC) pre
pared by apheresis during 5-day storage. Material and Methods: In one group
, 56 PC were prepared by blood cell separator CS-3000 Plus (Baxter GmbH) an
d the collecting chamber PLT 30(TM) With the Omnix(TM) system; in the secon
d group, 56 PC were prepared by blood cell separator AS-104 (Fresenius AG).
In order to assess the platelet function of PC, the following parameters h
ave been investigated at the day of donation and after 48-hour and 120-hour
storage: platelet reactivity according to the Grotemeyer method, index of
platelet aggregation induced by ADP and collagen, respectively, and platele
t count. Results: Due to an elevated number of hyperaggregable platelets in
PC separated with the CS-3000 Plus, platelet reactivity of PC in case of s
eparation with the CS-3000 Plus was higher (1.46 +/- 0.9) compared to that
of PC separated with the AS-104 (1.20 +/- 0.40; p = 0.064). The collagen-in
duced platelet aggregation after 48-hour storage was significantly higher i
n PC obtained by the AS-104 separator compared to that of the CS-3000 Plus
device (1.54 +/- 0.36 versus 1.45 +/- 0.36; p < 0.018). With an increase of
63.7%, the level of ADP-induced aggregation was higher in PC collected wit
h the AS-104 than that found in PC obtained by the CS-3000 Plus (34.3%; p =
0.018). With both methods, ADP-induced aggregation only occurred on the da
y of donation. In PC prepared by AS-104, the reduction of platelets during
5-day storage was 133 x 10(3)/mu l (11%) compared with 294 x 10(3)/mu l (21
%) in PC generated by the CS-3000 Plus. The volume of PC showed also differ
ences between the two methods. Conclusion: This investigation demonstrates
the necessity to define quality characteristics for PC regarding platelet f
unction in order to optimize the separation process.