C. Osterlund et al., Low number of Y-chromosome deletions in infertile azoospermic men at a Swedish andrology centre, INT J ANDR, 23(4), 2000, pp. 225-229
Recent studies have strongly indicated that at least three regions [azoospe
rmia factor (AZF) a-c] on the long arm of the Y-chromosome code for factors
involved in spermatogenesis. In order to reveal the prevalence of microdel
etions in these regions in a Swedish population, 192 men consecutively refe
rred to our andrology unit due to infertility and showing oligozoospermia (
n=53) or azoospermia (n=139) but no obstruction or hormonal disturbances, w
ere investigated. For this study we used a multiplex polymerase chain react
ion (PCR) method including 13 pairs of primers divided into five different
primer mixes. It was found that four men, all with azoospermia, had deletio
ns including part of the AZFb region and probably the entire AZFc region. T
estis biopsies showed different morphology ranging from absence of germ cel
ls to hypospermatogenisis. Of special interest was one patient that was fir
st investigated 10 years ago due to primary infertility and oligozoospermia
. Today he has developed azoospermia. It is concluded that the number of pa
tients with microdeletions on the Y chromosome is rather low (less than 3%
in highly selected azoospermic men) in our study compared to a number of ot
her studies in which a 1-55% incidence have been reported. It is possible t
hat ethnic differences, selection criteria and methodological aspects can c
ontribute to the difference between the present and previous studies.