The human paraoxonase Gln-Arg192 (Q/R) polymorphism in Turkish patients with coronary artery disease

It has been suggested that a Q/R (Gln192Arg) polymorphism of paraoxonase (P ON) might be associated with the predisposition to coronary artery disease (CAD). Therefore, we studied the human paraoxonase gene (PON1) polymorphism in Turkish patients with CAD by polymerase chain reaction/restriction frag ment length polymorphism (PCR/RFLP). This polymorphism was determined in 96 CAD patients and in 105 control subjects. The frequencies of the QQ, QR, a nd RR genotypes were found as 36.5, 52.0, and 11.5% in CAD patients and 48. 6, 41.0, and 10.4% in control subjects, respectively. The QR genotype was t he most common in the patient group, whereas the QQ genotype was more frequ ent in individuals without CAD. Frequency of the R allele was higher among CAD patients compared to controls (38.5% versus 31%). However, neither the genotype nor the allele distribution of the Gln92Arg polymorphism of PON1 w as statistically significantly different between the two groups (P>0.05). A lthough both systolic and diastolic blood pressure levels were slightly hig her in patients with the QQ genotype, there was no differences in regard to age, sex, serum triglyceride, total cholesterol or high-density lipoprotei n cholesterol among CAD patients with different PON1 Gln192Arg genotypes. I n summary, our results suggest that no association exists between the Gln19 2Arg polymorphism of paraoxonase and CAD in Turkish patients. (C) 2000 Else vier Science Ireland Ltd. All rights reserved.