Azospirillum species are plant-associated diazotrophs of the alpha subclass
of Proteobacteria. The genomes of five of the six Azospirillum species wer
e analyzed by pulsed-field gel electrophoresis. All strains possessed sever
al megareplicons, some probably linear, and 16S ribosomal DNA hybridization
indicated multiple chromosomes in genomes ranging in size from 4.8 to 9.7
Mbp. The nifHDK operon was identified in the largest replicon.