Roles of the heparin and low density lipid receptor-related protein-binding sites of protease nexin 1 (PN1) in urokinase-PN1 complex catabolism - ThePN1 heparin-binding site mediates complex retention and degradation but not cell surface binding or internalization
Rj. Crisp et al., Roles of the heparin and low density lipid receptor-related protein-binding sites of protease nexin 1 (PN1) in urokinase-PN1 complex catabolism - ThePN1 heparin-binding site mediates complex retention and degradation but not cell surface binding or internalization, J BIOL CHEM, 275(26), 2000, pp. 19628-19637
We have previously described thrombin (Th)-protease nexin 1 (PN1) inhibitor
y complex binding to cell surface heparins and subsequent low density lipid
receptor-related protein (LRP)-mediated internalization. Our present studi
es examine the catabolism of urinary plasminogen activator (uPA)-PN1 inhibi
tory complexes, which, unlike Th PN1 complexes, bind almost exclusively thr
ough the uPA receptor. In addition, the binding site in PN1 required for th
e LRP-mediated internalization of Th PN1 complexes is not required for the
LRP-mediated internalization of uPA.PN1 complexes. Thus, the protease moiet
y of the complex partially determines the mechanistic route of entry. Becau
se cell surface heparins are only minimally involved in the binding and int
ernalization of uPA.PN1 complexes, we then predicted that complexes between
uPA and the heparin binding-deficient PN1 variant, PN1(K7E), should be cat
abolized at the same rate as complexes formed with native PN1. Surprisingly
, the uPA.PN1(K7E) complexes were degraded at only a fraction of the rate o
f native complexes. Internalization studies revealed that both uPA.PN1(K7E)
and native uPA.PN1 complexes were initially internalized at the same rate,
but uPA.PN1(K7E) complexes were rapidly retro-endocytosed in an intact for
m. By examining the pH dependence of complex binding in the range of 4.0-7.
0, it was determined that the uPA.PN1 inhibitory complexes must specificall
y bind to endosomal heparins at pH 5.5 to be retained and sorted to lysosom
es. These studies are the first to document a role for heparins in the cata
bolism of SERPIN-protease complexes at a point further in the pathway than
cell surface binding, and this role may extend to other heparin-binding LRP
-internalized ligands.