Ra. Duffy et al., In vivo autoradiography of [H-3]SCH 39166 in rat brain: selective displacement by D1/D5 antagonists, J CHEM NEUR, 19(1), 2000, pp. 41-46
The purpose of this study was to examine the receptor occupancy of D1/D5 an
tagonists for D1-like dopamine receptors in rat brain using [H-3]SCH 39166,
a highly selective D1/D5 antagonist with low affinity for 5HT2 receptors.
A single concentration of triated SCH 39166 was administered to rats, with
or without competing doses of the D1/D5 antagonist SCH 23390 and unlabeled
SCH 39166, the D2-like antagonists haloperidol or the 5-HT2 antagonist keta
nserin. The bound radioactivity in the cortex, striatum. nucleus accumbens
and olfactory tubercle was then quantified using an in vivo autoradiographi
c procedure. The results indicated that [H-3]SCH 39166 was dose dependently
displaced by the D1/D5 antagonists in regions associated with both the nig
ro-striatal pathway and the mesolimbic dopamine pathway, particularly the n
ucleus accumbens. Neither haloperidol nor ketanserin displaced [H-3]SCH 391
66 in any of the regions examined. The data were compared with previously p
ublished data examining the in vivo binding of [H-3]SCH 39166 in rat brain
homogenates. The relative values obtained were comparable to values detecte
d in rat brain homogenates after in vivo binding of [H-3]SCH 39166. (C) 200
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