This study examines three methods of evaluating N2O flux and accumulat
ion in soil profile over a growing season under three soil management
regimes (fallow, fallow with manure addition and cropped to alfalfa).
Estimates of N2O flux were made based on measured soil atmosphere conc
entration gradients in the top 15 cm and compared to flux estimates ba
sed on ex situ cores and micro-meteorological measurements made in par
allel studies. All methods indicated strong seasonal trends relating t
o precipitation events. The amounts of N2O accumulating the profile de
creased in the order alfalfa < fallow < fallow/manure. The amounts of
N2O accumulating in the profile ranged from ambient (0.35 mu L L-1) to
490 mu L L-1. Diffusion of N2O to the lower profile was shown to prov
ide temporary storage of N2O and 2 thereby provide the opportunity for
further reduction to N-2 prior to efflux from the surface. In compari
ng the estimates of surface flux, all three methods were of the same o
rder of magnitude for the fallow site but profile-based estimates were
much lower and much higher for fallow/manure and alfalfa sites, respe
ctively. Differences were attributed to the location and timing of car
bon addition in each system.