Partitioning of xylanolitic complex from Penicillium janthinellum by an aqueous two-phase system

This work evaluates the influences of five parameters (pH, PEG molecular ma ss, PEG concentration, concentration of buffer K2HPO4-KH2PO4 and NaCl conce ntration) on xylanolitic complex partitioning produced by P. janthinellum i n aqueous two-phase systems, using a 2(5) factorial experimental design. A mathematical model to quantify the influence of these parameters was attain ed and statistically tested. The optimum point for total protein extraction was obtained under the following conditions: pN 7.0, PEG 10 000, 3.67% PEG , 10% potassium phosphate and 12.3% NaCl. The partition coefficient (K) val ue experimentally obtained was 5.25 and that predicted by the model was 5.8 9. (C) 2000 Eisevier Science B.V. All rights reserved.