Human mast cell migration in response to members of the transforming growth factor-beta family

Mast cells are known to accumulate at sites of inflammation, however, the c hemotaxins involved remain largely undefined. Transforming growth fastor-be ta (TGF-beta) isoforms regulate numerous cellular functions, including cell growth and differentiation, formation of extracellular matrix, and the imm une response. In this study we have compared the potency of different membe rs of the TGF-beta family as human mast cell chemotaxins, and analyzed the expression of TGF-beta binding proteins on human mast cells. We were able t o demonstrate that the maximal chemotactic response,vas attained at approxi mately 40 fM for the three TGF-beta isoforms, with TGF-beta 3 being more ef fective than TGF-beta 1 and TGF-beta 2 at this concentration. This effect w as observed in both the HMC-1 human mast cell line and in cultured primary mast cells. In addition, TGF-beta 1, TGF-beta 2, and less efficiently, TGF- beta 3 inhibited the proliferation of HMC-1 cells. The migratory response i s probably mediated through interaction with the TGF-beta serine/threonine type I and II receptors that were found to be expressed on the cells. No ex pression of TGF-beta type III receptor, endoglin, or the endothelial TGF-be ta type I receptor ALK-1 could be detected. These results provide evidence that TGF-beta isoforms are highly potent chemotaxins for human mast cells a nd can play an important role in the recruitment of mast cells in inflammat ory reactions.