Different regulation of cardiac and renal corticosteroid receptors in aldosterone-salt treated rats: Effect of hypertension and glucocorticoids

This study analysed the regulation of cardiac mineraloreceptor (MR) and glu coreceptor (GR) in aldosterone-salt treatment (AST). AST causes hypertensio n, left ventricle (LV) hypertrophy and decreases plasma corticosterone leve l, Ribonuclease protection assay and Western blot analysis showed a rise of MR mRNA (1.5- and 1.4-fold at day 15 and 30, respectively) and protein lev els (1.8- and 4.1-fold at day 30 and 60, respectively) in the LV, but not i n either the right ventricle (RV) or in kidney of treated rats. Addition of MR antagonist spironolactone (20 mg/kg/day) for 30 days failed to prevent these changes but was able to reduce AST-induced cardiac fibrosis, Similar hypertension-induced MR upregulations were observed in the LV of AngII-hype rtensive rats and of 12-week-old SAR when compared to 4-week-old prehyperte nsive SHR, AST also enhanced left ventricular GR mRNA (2.0- and 3.0-fold at day 7 and 15, respectively) and protein contents (2.0- and 1.7-fold at day 30 and 60, respectively). In contrast to MK, GR levels were also upregulat ed in both RV and kidney. Such an upregulation was equally observed at mRNA and protein levels in LV, RV and kidney after adrenalectomy (15 days) and was prevented in both tissues after glucocorticoid replacement (adrenalecto my + dexamethasone at 100 mu g/kg/day for 15 days), Therefore, MK level may be controlled by hemodynamical factors whereas that of GR depends upon glu cocorticoids level, (C) 2000 Academic Press.