An efficient extraction method from blood clots for studies requiring bothhost and viral DNA

The clot from blood is usually discarded after the collection of serum. Yet , it contains nucleated white blood cells and substantial serum. Here, we h ave compared four methods to enable quick and efficient extraction of human genomic and viral DNA from clotted blood. Two of these methods, a phenol-b ased in-house method and Tripure isolation reagent, only achieved a low pol ymerase chain reaction (PCR) yield. In contrast, the QIAamp blood kit and t he High Pure Viral Nucleic Acid kit were equally efficient, with similar se nsitivity to serum for extraction of viral DNA.