AMINOSULFONYL-7-N-(2-AMINOETHYL)AMINO-BENZOFURAZAN AS A NEW PRECOLUMNFLUORESCENCE DERIVATIZATION REAGENT FOR CARBOXYLIC-ACIDS (FATTY-ACIDSAND DRUGS CONTAINING A CARBOXYL MOIETY) IN LIQUID-CHROMATOGRAPHY

A new fluorescent reagent for carboxylic acids, 4-N,N-dimethylaminosul fonyl-7-N-(2-amino adiazole (DBD-ED) was synthesized from imethylamino sulfonyl-7-chloro-2,1,3-benzoxadiazole (DBD-Cl) and 1,2-diaminoethane. DBD-Cl is also a new compound described in this work for the first ti me. The applicability of DBD-ED as a precolumn derivatization reagent in liquid chromatography (LC) was examined. DBD-ED reacted with satura ted fatty acids and non-steroidal anti-inflammatory drugs (NSAIDs) in the presence of triphenylphosphine and 2,2'-dipyridyl disulfide at roo m temperature for 30 min for NSAIDs and 1 h for fatty acids to give fl uorescent products. The reaction solutions were subjected to LC and th e derivatized products were detected spectrofluorimetrically at 560 nm with excitation at 450 nm. The detection limits were in the fmol rang e.