Biological variables in non-small cell lung cancer: comparison between immunocytochemical determination on fine needle aspirates from surgical specimens and immunohistochemical determination on tissue sections
C. Bozzetti et al., Biological variables in non-small cell lung cancer: comparison between immunocytochemical determination on fine needle aspirates from surgical specimens and immunohistochemical determination on tissue sections, LUNG CANC, 29(1), 2000, pp. 33-41
A number of biological and predictive markers of non-small cell lung cancer
(NSCLC) have been sought, but these have so far been mainly evaluated on s
urgically resected specimens. Given that fine needle aspiration biopsy (FNA
B) is being increasingly used in the diagnosis of NSCLC, its application co
uld be extended to the immunocytochemical detection of biological parameter
s at the time of diagnosis before surgery. In order to assess the reliabili
ty of estimating biological markers on fine needle aspirates (FNAs) from NS
CLC, the aim of this study was to compare Ki67 growth fraction. p53 and bcl
-2 protein expression as revealed by the immuncytochemical assessement of F
NAs obtained from surgical samples with the immunohistochemical results obt
ained from the corresponding histological sections. FNAs were performed on
surgical specimens obtained from 29 NSCLC patients. Ki67, p53 and bcl-2 wer
e cytologically and histologically evaluable in respectively 25, 27 and 19
cases. Concordance between FNAs and corresponding paraffin sections was 84%
for Ki67. 93% for p53 and 95% for bcl-2. All of the specimens whose biolog
ical parameters were studied by immunocytohistochemistry also underwent flo
w cytometric DNA analysis of FNAs taken from fresh surgical specimens. Of t
he 29 cases, 22 were aneuploid and seven diploid. The S-phase fraction (SPF
) was evaluable in 62% of cases. Comparison of SPF results on FNAs with Ki6
7 values evaluated on the corresponding histologic and cytologic specimens,
revealed a significant correlation only with histology. Good reproducibili
ty was also found in relation to the immunocytochemical results obtained on
FNAs from different areas of the same tumour, showing that tumour heteroge
neity does not affect the method. The concordance between the immunocytoche
mical and immunohistochemical results suggests that FNAB may be a reliable
procedure for the biological characterization of NSCLC. Given its limited i
nvasiveness, FNAB could be used in vivo for the preoperative assessement of
biological parameters in patients with operable or metastatic NSCLC. (C) 2
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