Structure of a natural microbial community in a nitroaromatic contaminatedgroundwater is altered during biodegradation of extrinsic, but not intrinsic substrates
P. Wikstrom et al., Structure of a natural microbial community in a nitroaromatic contaminatedgroundwater is altered during biodegradation of extrinsic, but not intrinsic substrates, MICROB ECOL, 39(3), 2000, pp. 203-210
This study demonstrates microbial community changes over time in a nitroaro
matic-contaminated groundwater upon amendment with hydrocarbons previously
unknown to the microbial community (extrinsic) and hydrocarbons previously
known to the microbial community (intrinsic). Sealed flasks, shaken and inc
ubated at 25 degrees C, containing contaminated groundwater and salts were
amended twice with extrinsic hydrocarbons including phenol, benzoic acid, a
nd naphthalene, and intrinsic hydrocarbons including 2,4-dinitrotoluene (2,
4-DNT) and para-nitrotoluene (p-NT). Microbial growth, biodegradation, and
community structure changes measured by random amplified polymorphic DNA (R
APD) and quantitative PCR (qPCR) targeting catechol-2,3-dioxygenase (C230)
genes were monitored over time. All amended substrates were biodegraded aft
er both substrate amendments except for 2,4-DNT, which was only partially d
egraded after the second amendment. Unique microbial communities were devel
oped in flasks amended with phenol, benzoic acid, and naphthalene. However,
in the flasks amended with intrinsic hydrocarbons the microbial community
remained similar to the unamended control flasks. The relative amount of C2
30 genes detected by qPCR correlated with the biodegradation of phenol and
naphthalene but not with 2,4-DNT. The results showed that a selection for m
icroorganisms capable of catabolizing extrinsic hydrocarbons naturally and
initially present in the nitroaromatic-contaminated groundwater occurred. H
owever, growth-linked biodegradation of added intrinsic hydrocarbons was no
t selective.