Citation
P. Vinas et al., Flow-injection fluorimetric determination of thiamine in pharmaceutical preparations, MIKROCH ACT, 134(1-2), 2000, pp. 83-87
Citations number
31
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
MIKROCHIMICA ACTA
ISSN journal
00263672
→ ACNP
Volume
134
Issue
1-2
Year of publication
2000
Pages
83 - 87
Database
ISI
SICI code
0026-3672(2000)134:1-2<83:FFDOTI>2.0.ZU;2-G
Abstract
A new fluorimetric procedure for the determination of thiamine using flow i
njection analysis is proposed. The method is based on the derivatization re
action of the primary amine group with o-phthalaldehyde in the presence of
2-mercaptoethanol using fluorimetric detection. The calibration graph based
on peak area was linear in the range 0.2-6 ng mL(-1). The detection limit
was close to 0.1 ng mL(-1). The method was applied to the determination of
the vitamin in commercial pharmaceutical preparations.