Substrate mimetics and freezing strategy: A useful combination that broadens the scope of proteases for synthesis

GRAPHICS We present an irreversible and efficient protease based method for peptide synthesis which occurs independently of the primary specificity of protease s and also without proteolytic side reactions, The key feature of this appr oach is the combination of the substrate mimetics strategy with frozen stat e enzymology. Model reactions catalyzed by several proteases qualify this a pproach as a powerful concept in the direction of a more universal applicat ion of proteases as biocatalysts for peptide ligation.