The Balearic archipelago (Spain) is composed of three major islands, M
ajorca, Minorca, and Ibiza. Majorca is the largest and most populous i
sland. Minorca is the second largest in area and third in population,
and Ibiza is the smallest of the three and has the second largest popu
lation. Ibiza also shows differences from the other two islands in its
landscape, vegetation, and especially origins of the founding settlem
ents. The three islands also have been settled by people of different
cultures. We have carried out a genetic analysis at the level of mtDNA
RFLPs in these populations and found that the three populations are g
enetically structured, with differences in the RFLP haplotype frequenc
ies reflecting the different population substrata of the three islands
. In this structure Ibiza is genetically more different from Majorca a
nd Minorca. In addition, we found a new haplotype in Minorca, named ha
plotype 150, which seems to originate from haplotype 56.