Regional differences in neurogenic signal transduction pathway of cholera toxin-induced fluid, electrolyte and serotonin accumulation in the porcine jejunum
P. Berggreen et al., Regional differences in neurogenic signal transduction pathway of cholera toxin-induced fluid, electrolyte and serotonin accumulation in the porcine jejunum, PHARM TOX, 86(6), 2000, pp. 270-275
Serotonin, acetylcholine and substance P are mediators involved in the secr
etory response to cholera toxin in the small intestine. The aim of this stu
dy was to investigate the regional difference in the effect of a serotonin
receptor type 3 antagonist (ondansetron), a nicotinic receptor antagonist (
hexamethonium), and a substance P antagonist (the neurokinin receptor type
1 antagonist, CP 99,994) on the cholera toxin-induced fluid accumulation in
the porcine jejunum. A dose-range of cholera toxin (0.32-56.00 mu g/loop)
was instilled for 4 hr in ligated loops in two regions of the proximal jeju
num in 6-8-week-old pigs. Ondansetron (200 mu g/kg), hexamethonium (10 mg/k
g), CP 99,994 (1 mg/kg), or saline alone (control) were given intravenously
10 min, before cholera toxin instillation. Cardiovascular parameters, bloo
d gas data, net fluid accumulation, serotonin and electrolyte concentration
in the accumulated fluid were measured. Cardiovascular and blood gas param
eters were within the normal range in all treatments. The apparent maximal
response in fluid accumulation was reduced 20% in case of ondansetron, and
by 33% using CP 99,994 in the aboral region compared to control, whereas no
effect was observed in the oral region. Hexamethonium reduced the apparent
maximal secretory response in both the oral and aboral regions by 45%. Non
e of the treatments with antagonists changed the luminal content of seroton
in or the electrolyte concentrations in the accumulated fluid. The results
demonstrate that the involvement of serotonin receptor type 3 and neurokini
n type 1 receptors in the transductional pathway of cholera toxin-induced f
luid accumulation vary significantly within the jejunum, while the choliner
gic (nicotinic) transmission plays an even role.