Estimating the growth of Corynebacterium paurometabolum in heterogeneous media

The nematocidic agent Corynebacterium paurometabolum was cultured in autocl aved suspensions of torula yeast. To determine the growth of the microorgan ism in the suspensions, a standard addition technique was developed; which could discriminate the optical density of the bacterial biomass and the sus pended substrate absorbance. Consequently. the correction absorbance curves for each suspension and microorganism growth curves were obtained. The sta tistical analysis of the humid cell weight variable in the interval studied showed that a torula yeast suspension at 3% (w/v) is the most efficient ch oice for biomass production. (C) 2000 Elsevier Science Ltd. All rights rese rved.