B. Ohlsson et al., Continuous infusion of cholecystokinin leads to down-regulation of the cholecystokinin-A receptor in the rat pancreas, SC J GASTR, 35(6), 2000, pp. 612-618
Background: Infusion of sulphated cholecystokinin-8 (CCK-8S) in rats transi
ently increased the proliferation of pancreatic acinar cells, whereas the C
CK-A receptor antagonist devazepide decreased such proliferation. This effe
ct ceased after 3 days. CCK-8S or devazepide injected twice daily induced a
persistent effect on the cell proliferation involving the major cells of t
he exocrine pancreas. The aim of this study was to examine the effect of co
ntinuous infusion of CCK-8S and devazepide on CCK-A receptor gene expressio
n. Methods: Male Sprague-Dawley rats received subcutaneous continuous infus
ion of 5 mu g/kg/h CCK-8S, 200 mu g/kg/h devazepide, or 1% bovine serum alb
umin (BSA) by means of osmotic minipumps; The rats were killed after 3 days
; 1 h before bring killed they received 5-broino-2-deoxyuridine (BrdU) intr
aperitoneally. Plasma was collected for analysis of CCK. The pancreas was d
issected, and indirect immunofluorescence for BrdU and CCK-A receptor was p
erformed. In situ hybridization to CCK-A receptor mRNA was performed for ex
amination and semiquantification of receptor gene expression. Results: Cont
inuous infusion of CCK-8S led to a sixfold increase in plasma CCK and a 40%
increase in pancreatic weight. Devazepide did not affect the CCK level but
decreased the pancreatic weight by 24% compared with BSA-infused rats. The
BrdU labeling indicated that CCK-8S had no effect on cell proliferation. I
mmunofluorescence for the CCK-A receptor showed a decreased labeling intens
ity after CCK-8S infusion. The mean optical density of in situ hybridizatio
n labeling of the sections from CCK-8S-treated rats was decreased to 37% +/
- 3% of that in controls. Devazepide did not affect the CCK-A receptor gene
expression. Conclusions: Continuous stimulation of the CCK-B receptor led
to a downregulation of the receptor gene expression in pancreatic acinar ce
lls and decreased labeling of the receptor at immunohistochemistry. The res
ults suggest that down-regulation of the receptor is a protective mechanism
against overstimulation.