Cytokine profiles in seronegative volunteers immunized with a recombinant canarypox and gp120 prime-boost HIV-1 vaccine

Citation
S. Sabbaj et al., Cytokine profiles in seronegative volunteers immunized with a recombinant canarypox and gp120 prime-boost HIV-1 vaccine, AIDS, 14(10), 2000, pp. 1365-1374
Citations number
46
Categorie Soggetti
Immunology
Journal title
AIDS
ISSN journal
02699370 → ACNP
Volume
14
Issue
10
Year of publication
2000
Pages
1365 - 1374
Database
ISI
SICI code
0269-9370(20000707)14:10<1365:CPISVI>2.0.ZU;2-P
Abstract
Objectives: To study memory T cell proliferative responses and cytokine pro files induced in HIV-1 seronegative volunteers immunized with a live recomb inant canary-pox vector expressing HIV-1 antigens (ALVAC-HIV) and boosted w ith a recombinant gp120 subunit vaccine. Design: HIV-specific T cell proliferative responses and cytokines were meas ured 2 weeks after vaccination. Cytokines secreted by T helper 1 cells (Th1 ) [interleukin (IL)-2 and interferon-gamma (IFN-gamma)] and T helper 2 (Th2 ) cells (IL-4, IL-5, IL-6, and IL-10) were assessed both at the mRNA and th e protein level. Methods: Peripheral blood mononuclear cells (PBMC) were stimulated in vitro with HIV antigens. Subsequently, T cell proliferation was measured in a st andard lymphoproliferation assay; secreted cytokines were measured using an enzyme-linked immunosorbent assay and upregulation of cytokine mRNA was me asured using reverse transcriptase polymerase chain reaction. Results: All individuals who had received ALVAC-HIV followed by the protein vaccine exhibited HIV-l-specific T cell proliferative responses. Moreover, the PBMC of all prime-boost vaccinated individuals produced detectable IFN -gamma and IL-10 in response to stimulation with HIV-1 envelope glycoprotei n antigens; 83% also had detectable levels of IL-2 and IL-6, 71% had detect able levels of IL-4, and 86% had detectable levels of IL-5. Conclusions: These data indicate that this vaccination regimen was inducing both Th1- and Th2-type responses to HIV-1 envelope antigens. This prime-bo ost vaccination approach elicited T cell help for the generation of cytotox ic T lymphocyte responses as well as help for antibody production and so pr omises to generate a broad HIV-l-specific immune response. (C) 2000 Lippinc ott Williams & Wilkins.