7C Gold urinary assay of neural thread protein in Alzheimer's disease

Neural thread proteins (NTP) are recently characterized brain proteins, of which the 41 kD AD7C-NTP protein is selectively elevated in brain, cerebros pinal fluid, and urine in Alzheimer's disease (AD). AD7C-NTP is associated with the pathological changes of AD, and the overexpression of the AD7CNTP gene in transfected cells in culture induces cell death with sprouting. Usi ng a newly developed gold particle monoclonal anti-AD7C-NTP based liquid ph ase immunoassay (7C Gold), AD7C-NTP was measured in 58 cases of AD and 31 h ealthy normal controls. The mean 7C Gold migration measurement in the AD gr oup was significantly higher than the control group (p<0.005). 7C Gold migr ation levels of greater than 125 units were found in 91.4% of AD cases, and in 3.2% of controls. 7C Gold levels above 139 were found in 86.2% of AD ca ses and 0% of controls. This data further validates AD7C-NTP as a biochemic al marker for AD, and indicates that the 7C Gold test in urine is an accura te method to determine AD7C-NTP levels in AD.