Lf. Del Aguila et al., Muscle damage impairs insulin stimulation of IRS-1, PI 3-kinase, and Akt-kinase in human skeletal muscle, AM J P-ENDO, 279(1), 2000, pp. E206-E212
Citations number
43
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
Physiological stress associated with muscle damage results in systemic insu
lin resistance. However, the mechanisms responsible for the insulin resista
nce are not known; therefore, the present study was conducted to elucidate
the molecular mechanisms associated with insulin resistance after muscle da
mage. Muscle biopsies were obtained before (base) and at 1 h during a hyper
insulinemic-euglycemic clamp (40 mU . kg(-1) . min(-1)) in eight young (age
24 +/- 1 yr) healthy sedentary (maximal O-2 consumption, 49.7 +/- 2.4 ml .
kg(-1) . min(-1)) males before and 24 h after eccentric exercise (ECC)-ind
uced muscle damage. To determine the role of cytokines in ECC-induced insul
in resistance, venous blood samples were obtained before (control) and 24 h
after ECC to evaluate ex vivo endotoxin-induced mononuclear cell secretion
of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-1 beta. G
lucose disposal was 19% lower after ECC (P< 0.05). Insulin-stimulated insul
in receptor substrate (IRS)-1 tyrosine phosphorylation was 45% lower after
ECC (P< 0.05). Insulin-stimulated phosphatidylinositol (PI) 3-kinase, Akt (
protein kinase B) serine phosphorylation, and Akt activity were reduced 34,
65, and 20%, respectively, after ECC (P< 0.05). TNF-alpha, but not IL-6 or
IL-1 beta production, increased 2.4-fold 24 h after ECC (P< 0.05). TNF-alp
ha production was positively correlated with reduced insulin action on PI 3
-kinase (r = 0.77, P = 0.04). In summary, the physiological stress associat
ed with muscle damage impairs insulin stimulation of IRS-1, PI 3-kinase, an
d Akt-kinase, presumably leading to decreased insulin-mediated glucose upta
ke. Although more research is needed on the potential role for TNF-alpha in
hibition of insulin action, elevated TNF-alpha production after muscle dama
ge may impair insulin signal transduction.