Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli

Authors
Henne, A Schmitz, RA Bomeke, M Gottschalk, G Daniel, R
Citation
A. Henne et al., Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli, APPL ENVIR, 66(7), 2000, pp. 3113-3116
Citations number
26
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
66
Issue
7
Year of publication
2000
Pages
3113 - 3116
Database
ISI
SICI code
0099-2240(200007)66:7<3113:SOEDLF>2.0.ZU;2-I
Abstract
Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clone s. Screening on triolein agar revealed 1 positive clone out of 730,000 clon es, and screening on tributyrin agar revealed 3 positive clones out of 286, 000 E. coli clones. Substrate specificity analysis revealed that one recomb inant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and character ized.