Detection of intracellular bacteria in the buds of Scotch fine (Pinus sylvestris L.) by in situ hybridization

Authors
Pirttila, AM Laukkanen, H Pospiech, H Myllyla, R Hohtola, A
Citation
Am. Pirttila et al., Detection of intracellular bacteria in the buds of Scotch fine (Pinus sylvestris L.) by in situ hybridization, APPL ENVIR, 66(7), 2000, pp. 3073-3077
Citations number
33
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
66
Issue
7
Year of publication
2000
Pages
3073 - 3077
Database
ISI
SICI code
0099-2240(200007)66:7<3073:DOIBIT>2.0.ZU;2-G
Abstract
Bacterial isolates were obtained from pine (Pinus sylvestris L.) tissue cul tures and identified as Methylobacterium extorquens and Pseudomonas synxant ha. The existence of bacteria in pine buds was investigated by 16S rRNA in situ hybridization. Bacteria inhabited the buds of every tree examined, pri marily colonizing the cells of scale primordia and resin ducts.