Physiological and growth responses of Thalassia testudinum to environmentally-relevant periods of low irradiance

Turtle grass (Thalassia testudinum) was exposed to either Control (6 weeks of unscreened irradiance), Long Period (3 weeks of screened [8% of unscreen ed] followed by 3 weeks of unscreened irradiance), or Short Period (alterna ting 7-day periods of 8% and 100% unscreened irradiance for a total of 6 we eks) treatment. Six-week experiments were conducted during late winter (Feb ruary-March) and summer (May-July), and indices of carbon (C) and nitrogen (N) metabolism and growth were monitored. The uptake of inorganic N (NH4+, [NO2- + NO3-]) and phosphorus (PO43-) by seagrasses and epiphytes was great er during the unscreened phases than during the screened phases of the trea tments. Soluble carbohydrate content of leaf tissue of T. testudinum declin ed during the screened phases of the experimental treatments and increased under the unscreened phases while levels in rhizome tissue did not vary sig nificantly. Leaf elongation rates of the Control and Short treatments follo wed similar patterns during both experiments, while rates under the Long tr eatment were depressed by an average of 18%. Shoot glutamine synthetase (GS ) activity also responded to light availability, decreasing immediately whe n the plants were screened and then increasing upon removal of the screens. The magnitude of the decrease in GS activity depended on season (greater d uring summer). Only GS activity differed significantly as a function of tre atment on the last (42nd) day of the experiments. Overall, the physiology o f T. testudinum was resistant to change and resilient following severe, per iodic light limitation on ecologically relevant, intermediate time scales. (C) 2000 Elsevier Science B.V. All rights reserved.