Optical biosensor study of ternary complex formation in a cytochrome P4502B4 system

The optical biosensor method was used for the revelation of ternary complex es, formed by the full-length NADPH-cytochrome P450 reductase (d-Fp) and cy tochromes P4502B4 (d-2B4) and b5 (d-b5) in the course of their interactions within the reconstituted d-2B4-containing system. Based on the lack of com petition between d-b5 and d-Fp for the binding sites on immobilized 2B4 (3) as well as on the analysis of data obtained in the three proteins' dissoci ation reactions, the possibility of formation of ternary complexes through the interactions between membranous hydrophobic fragments of proteins was s ubstantiated. All the complexes obtained were productive. (C) 2000 Academic Press.