In situ and in vitro evidence for stereoselective and carrier-mediated transport of monocarboxylic acids across intestinal epithelial tissue

The present study was designed to establish the significance of carrier-med iated transport in the intestinal absorption of monocarboxylic acids by exa mining the stereoselectivity of transepithelial transport of chiral monocar boxylic acids. The transport of L- and D-lactic acids was examined in vitro using rat intestinal tissue sheets and in situ by means of intra-jejunal a dministration, followed by measurement of the plasma concentration. Both th e absorptive and secretory transport of L-[C-14]lactic acid across the inte stinal epithelial tissues of rats was significantly greater than that of th e D-isomer. The secretory transport of the L-isomer was significantly great er than the absorptive transport, implying net transport in the secretory d irection. When L- and D-[C-14]lactic acids were administered to the rat jej unum, the absorption ratio of the L-isomer was lower than that of the D-iso mer at 15 min after administration. The concentration-dependence of absorpt ion for both L- and D-[C-14]lactic acids indicated the involvement of both saturable and nonsaturable processes. The saturable process showed a higher affinity and lower capacity for L-lactic acid compared with the D-isomer, while no significant difference between the isomers was observed in the non saturable process. The absorption of L-lactic acid was inhibited by chiral 2-hydroxymonocarboxylic acids in a stereoselective manner. Chiral monocarbo xylic acids were shown to cross the intestinal epithelial tissues and to be absorbed in a stereoselective manner after oral administration, suggesting the involvement of specific carrier-mediated transport mechanism(s) in the ir intestinal absorption in vivo.