Enhanced binding of aminoglycoside dimers to a "dimerized" A-site 16S rRNAconstruct

Authors
Tok, JBH Huffman, GR
Citation
Jbh. Tok et Gr. Huffman, Enhanced binding of aminoglycoside dimers to a "dimerized" A-site 16S rRNAconstruct, BIOORG MED, 10(14), 2000, pp. 1593-1595
Citations number
21
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
10
Issue
14
Year of publication
2000
Pages
1593 - 1595
Database
ISI
SICI code
0960-894X(20000717)10:14<1593:EBOADT>2.0.ZU;2-M
Abstract
In this work, we investigated the binding of a series of dimeric aminoglyco side molecules to (i) a 27 nt A-site 16S rRNA construct, and (ii) an artifi cially grafted 46 nt 'dimerized' A-site 16S rRNA construct. It was observed that the dissociation constants of dimeric aminoglycosides to the dimerize d A-site 16S rRNA construct can achieve up to approximately 19-fold enhance ment compared to the monomeric aminoglycoside molecules. (C) 2000 Elsevier Science Ltd. All rights reserved.