Binding interactions of vancomycin tracers with a bacterial cell wall peptidoglycan analogue

Binding interactions between several vancomycin tracers and (N,N'-diacetyl) KDADA in solution were evaluated in a competition format using a surface pl asmon resonance instrument. Tracers derivatized from the carboxy terminus o r the N-vancosaminyl sugar moiety of vancomycin bind the peptide with an af finity similar to that of underivatized vancomycin. In contrast, N-methylle ucyl derivatized vancomycin tracers bind the peptide with a reduced affinit y relative to vancomycin. (C) 2000 Elsevier Science Ltd. All rights reserve d.