Comparison between two processes for the enzymatic synthesis of tri-docosahexaenoylglycerol in a solvent-free medium

Authors
Borg, P Girardin, M Rovel, B Barth, D
Citation
P. Borg et al., Comparison between two processes for the enzymatic synthesis of tri-docosahexaenoylglycerol in a solvent-free medium, BIOTECH LET, 22(9), 2000, pp. 777-781
Citations number
14
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
9
Year of publication
2000
Pages
777 - 781
Database
ISI
SICI code
0141-5492(200005)22:9<777:CBTPFT>2.0.ZU;2-K
Abstract
The enzymatic synthesis of homogeneous tri-docosahexaenoylglycerol from gly cerol and ethyl docosahexaenoate in a solvent-free medium was achieved usin g Novozym SP 435 (immobilized lipase from Candida antarctica). Two processe s were tested: a constant stirred tank reactor with N-2 bubbling through an d a constant stirred tank reactor under vacuum. The first experimental appa ratus was clearly found to be better than the other, giving 100% (w/w) conv ersion after 10 h.